Practical skills in biomolecular sciences /
Rob Reed ... [et al.].
- 2nd ed.
- Harlow, England ; New York : Pearson/Prentice Hall, 2003.
- xiv, 538 p. : ill. ; 27 cm.
Includes bibliographical references and index.
Machine generated contents note: List of Boxes vii -- Preface to the Second Edition ix -- For the Student xi -- Acknowledgements xii -- List of Abbreviations xiii --Study and examination skills -- 1 The importance of transferable skills 3 -- 2 Managing your time 7 -- 3 Working with others 12 -- 4 Taking notes from lectures and texts 16 -- 5 Revising 21 -- 6 Assessments and exams 25 -- 7 Preparing your curriculum vitae 33 --Information technology and library resources -- 8 Finding and citing published information 41 -- 9 Evaluating information 47 -- 10 Using the Internet and World Wide Web 53 -- 11 Bioinformatics - Internet resources 61 -- 12 Using spreadsheets 68 -- 13 Word processors, databases and other packages 74 --Communicating information -- 14 Organising a poster display 83 -- 15 Giving a spoken presentation 87 -- 16 General aspects of scientific writing 93 -- 17 Writing essays 99 -- 18 Reporting practical and project work 103 -- 19 Writing literature surveys and reviews 108 --Fundamental laboratory techniques -- 20 Your approach to practical work 113 -- 21 Health and safety 117 -- 22 Working with liquids 121 -- 23 Basic laboratory procedures 128 -- 24 Principles of solution chemistry 138 -- 25 pH and buffer solutions 146 -- 26 Introduction to microscopy 154 -- 27 Setting up and using a light microscope 159 --The investigative approach -- 28 Making and recording measurements 169 -- 29 SI units and their use 176 -- 30 Scientific method and design of experiments 182 -- 31 Project work 191 --Handling cells and tissues -- 32 Sterile technique and microbial culture 197 --33 Isolating and identifying microbes 205 -- 34 Working with animal and plant tissues and cells 218 -- 35 Culture systems and growth measurement 225 -- 36 Homogenisation and fractionation of cells and tissues 237 --Analytical techniques -- 37 Calibration and its application to quantitative analysis 245 -- 38 Immunological methods 250 -- 39 Radioactive isotopes and their uses 258 -- 40 Light measurement 267 -- 41 Basic spectroscopy 271 -- 42 Advanced spectroscopy and spectrometry 280 -- 43 Centrifugation 289 -- 44 Chromatography - separation methods 296 -- 45 Chromatography - detection and analysis 307 -- 46 Principles and practice of electrophoresis 313 -- 47 Advanced electrophoretic techniques 324 -- 48 Electroanalytical techniques 331 --Assaying biomolecules and studying metabolism -- 49 Analysis of biomolecules - fundamental principles 343 -- 50 Assaying amino acids and proteins 347 -- 51 Assaying lipids 353 -- 52 Assaying carbohydrates 359 -- 53 Assaying nucleic acids and nucleotides 364 -- 54 Protein purification 370 -- 55 Enzyme studies 378 -- 56 Membrane transport processes 390 -- 57 Photosynthesis and respiration 397 --Genetics -- 58 Mendelian genetics 411 -- 59 Bacterial and phage genetics 418 -- 60 Molecular genetics I - fundamental principles 427 -- 61 Molecular genetics II - PCR and related applications 437 -- 62 Molecular genetics III - genetic engineering techniques 443 --Analysis and presentation of data -- 63 Using graphs 453 -- 64 Presenting data in tables 462 -- 65 Hints for solving numerical problems 465 -- 66 Descriptive statistics 475 -- 67 Choosing and using statistical tests 485 --Answers to study exercises 497 -- Index 529.